Primer

Part:BBa_K5232007

Designed by: Dr. Ken Komiya (Extra Adviser)   Group: iGEM24_UTokyo   (2024-09-28)

Usage and Biology

The amplification product of the second step of the multistep-SDA reaction.

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Figure 1.
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Figure 2.

By using a template with TAMRA modification at the 3' end, amplification was confirmed within 200 min of the observation. For step 123, amplification was confirmed when the target concentration was 100 pM or higher. Amplification of NCs was not confirmed during the observation time.

Sequence and Features No part name specified with partinfo tag.

References

Komiya, K., Noda, C. & Yamamura, M. (2024). Characterization of Cascaded DNA Generation Reaction for Amplifying DNA Signal.New Gener. Comput. 42, 237-252. https://doi.org/10.1007/s00354-024-00249-2


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